Coding
Part:BBa_K1321346:Design
Designed by: Chris N Micklem Group: iGEM14_Imperial (2014-10-08)
CBDclos fused to sfGFP in RFC 25
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 316
Design Notes
Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.
Source
CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.