Coding

Part:BBa_K1321346:Design

Designed by: Chris N Micklem   Group: iGEM14_Imperial   (2014-10-08)


CBDclos fused to sfGFP in RFC 25


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 316


Design Notes

Codon optimised for E.coli. Once the individual parts were confirmed in Freiburg format, we cloned them using the AgeI and NgoMIV site, creating a scar that also functions as a small linker.

Source

CBDclos was requested from the registry, as it was submitted by the Freiburg team as BBa_K863111. Our sfGFP part was an improvement of BBa_I746909, wherein Freiburg sites were added. Please see BBa_K1321337 for more info on this part.

References